Table II.
Line | Light-Grown
Seedlings
|
Dark-Grown Seedlings
|
||
---|---|---|---|---|
−GA | +GA | −GA | +GA | |
Hypocotyl length | mm | |||
25-1 | 2.4 ± 0.09 | 4.4 ± 0.10 | 20.1 ± 0.29 | 19.0 ± 0.48 |
25-2 | 4.2 ± 0.10 | 5.1 ± 0.37 | 21.0 ± 0.29 | 18.3 ± 0.42 |
25-3a | 3.8 ± 0.10 | 4.6 ± 0.21 | 20.2 ± 0.40 | 22.1 ± 0.50 |
25-24 | 2.6 ± 0.09 | 3.7 ± 0.10 | 20.5 ± 0.37 | 20.2 ± 0.39 |
Wild type | 2.0 ± 0.10 | 4.3 ± 0.20 | 21.2 ± 0.34 | 19.9 ± 0.28 |
Root length | ||||
25-1 | 17.2 ± 0.70 | 20.6 ± 0.55 | 13.7 ± 0.34 | 12.9 ± 0.48 |
25-2 | 18.0 ± 0.64 | 18.4 ± 0.59 | 14.2 ± 0.40 | 13.4 ± 0.43 |
25-3a | 17.5 ± 0.91 | 22.9 ± 0.56 | 17.8 ± 0.39 | 15.8 ± 0.35 |
25-24 | 19.0 ± 0.56 | 20.0 ± 0.65 | 15.0 ± 0.43 | 13.8 ± 0.30 |
Wild type | 18.7 ± 0.77 | 19.1 ± 0.67 | 13.7 ± 0.42 | 13.7 ± 0.30 |
Transgenic and wild-type seeds were germinated on vertical plates, as shown in Figure 2A. These seedlings were grown under either 16 h of light or total darkness with (+) or without (−) GA3 (10 μm) in the medium. Data are means ± se (n < 30).
The integration of the transgene in line 25-3 resulted in a recessive albino mutation; therefore, hemizygous transgenic plants of line 25-3 were used to generate data for this and the following tables.