Table 10.

DNA sequencing reaction conditions

Conditions for All Exons Except Exon 1	Conditions for Exon 1
30 cycles: •96°C for 10 sec •50°C for 5 sec •60°C for 4 min	30 cycles: •96°C for 10 sec •60°C for 1 min Hold at 4°C
Hold at 4°C