Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HaploPrep reaction master mix
| Components | Volume in Each Reaction |
|---|---|
| Hybridization buffer H | 15 µl |
| HaploPrep Extraction Probe: 2DL2-999T or 2DL3-1316T |
2 µl |
| Water | 8 µl |
| Genomic DNA | 5 µl (added at a later step) |
The volume for a single reaction is 25 µl without the DNA added so multiple the number of reactions desired by 25 to determine how much reaction master mix to make. Always make more than you need to account for losses during pipetting.
