Table.
Quantitative RT-PCR Analysis of 4 Genes on Chromosome 11q13 That Are Underexpressed at Baseline in ACC and Differentially Expressed in NCI-H295R Cells at 3 Days After Decitabine (1.0μM) Treatment
| Gene Symbol | Gene Name | Fold Change in Gene Expression in ACCa | Change in Gene Expression at 3 Days After Decitabine (1.0μM) Treatment, %b | P valuec |
|---|---|---|---|---|
| DDB1 | Damage-specific DNA binding protein 1 | −2.85 | −20.9 | .02 |
| NDUFS8 | NADH dehydrogenase (ubiquinone) Fe-S protein 8 | −2.00 | 33.1 | <.001 |
| PRDX5 | Peroxiredoxin 5 | −2.40 | 50.3 | .006 |
| TM7SF2 | Transmembrane 7 superfamily member 2 | −4.28 | −42.0 | .04 |
Abbreviations: ACC, adrenocortical carcinoma; NADH, nicotinamide adenine dinucleotide; RT-PCR, reverse transcription-polymerase chain reaction.
a
From the microarray analysis by Fernandez-Ranvier et al.12
b
Based on RT-PCR performed herein in NCI-H295R cells (ATCC, Rockville, Maryland).
c
Two-tailed t test.