Table 1. Percentage of LNT, fucosylation and sialylation in the free HMOs in milk samples collected from fifteen mothers who delivered pre-term.
ID = premature mother number, GA = gestational age at birth, PMA = postmenstrual age (the gestational age at birth plus the chronological age in weeks at the time of milk collection). %Fuc and %Sia are the percent fucosylation and sialylation, respectively, normalized to the total HMO abundance per sample. Percent monofucosylation, difucosylation, trifucosylation and tetrafucosylation are in columns marked as %Mono Fuc, %Di Fuc, %Tri Fuc, %Tetra Fuc correspondingly and refer to the percentages of HMOs with one, two, three and four fucoses, respectively. The percent abundance of 2′-fucosyllactose, an oligosaccharide secretor status marker bearing the alpha-1,2-fucose, is shown in the column %2′-FL, along with its observed neutral mass from nano-LC Chip/TOF MS and mass error in parts per million (ppm). ND=not detected.
| ID | BW (GM) |
GA (WEEKS) |
PMA (WEEKS) |
% SIA |
% FUC |
% MONO FUC |
% DI FUC |
% TRI FUC |
% TETRA FUC |
% 2′FL |
2′ FL MASS |
2′ FL MASS ERROR, PPM |
3-FL | LNT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3P | 570 | 25 | 32 | 26.1% | 58.0% | 71.4% | 25.2% | 3.4% | 0.0% | 0.1% | 490.1898 | 0.204 | 0.14% | 21.2% |
| 4P | 1120 | 27 | 28 | 23.7% | 32.9% | 80.2% | 16.5% | 3.1% | 0.1% | 0.0% | 490.1899 | 0.408 | 0.03% | 43.1% |
| 31 | 27.9% | 26.2% | 83.4% | 14.8% | 1.8% | 0.0% | 0.1% | 490.1897 | 0.000 | 0.03% | 48.8% | |||
| 6P | 1170 | 27 | 31 | 8.4% | 52.8% | 84.0% | 14.8% | 1.2% | 0.0% | 5.6% | 490.1915 | 3.672 | ND | 35.0% |
| 33 | 7.6% | 61.2% | 75.9% | 21.3% | 2.5% | 0.3% | 7.4% | 490.1912 | 3.060 | ND | 26.0% | |||
| 34 | 6.2% | 71.6% | 79.8% | 16.6% | 3.3% | 0.3% | 19.1% | 490.1901 | 0.816 | 0.06% | 19.3% | |||
| 7P | 1450 | 30 | 31 | 3.6% | 30.0% | 87.3% | 12.5% | 0.3% | 0.0% | ND | ND | ND | 0.01% | 52.5% |
| 32 | 9.9% | 58.0% | 76.6% | 21.0% | 2.3% | 0.0% | 0.3% | 490.1891 | 1.224 | ND | 33.9% | |||
| 34 | 8.3% | 36.6% | 67.9% | 30.1% | 2.0% | 0.0% | 0.3% | 490.1883 | 2.856 | 0.06% | 51.5% | |||
| 36 | 17.3% | 34.1% | 75.6% | 20.3% | 4.1% | 0.0% | 0.7% | 490.1900 | 0.612 | ND | 35.9% | |||
| 9P | 1030 | 26 | 31 | 21.7% | 58.6% | 63.6% | 34.4% | 1.9% | 0.1% | 16.1% | 490.1903 | 1.224 | 0.04% | 13.1% |
| 10P | 920 | 28 | 30 | 16.3% | 26.1% | 67.1% | 30.0% | 2.4% | 0.1% | 0.6% | 490.1907 | 2.040 | ND | 28.9% |
| 32 | 6.2% | 62.5% | 87.6% | 10.9% | 1.5% | 0.1% | 16.3% | 490.1895 | 0.408 | ND | 26.9% | |||
| 34 | 3.9% | 72.5% | 86.3% | 12.5% | 1.1% | 0.1% | 28.4% | 490.1893 | 0.816 | ND | 20.6% | |||
| 36 | 6.5% | 30.7% | 60.0% | 37.7% | 2.2% | 0.1% | 3.9% | 490.1895 | 0.408 | ND | 46.3% | |||
| 13P | 570 | 24 | 28 | 17.9% | 49.5% | 55.0% | 41.2% | 3.5% | 0.3% | 7.9% | 490.1906 | 1.836 | ND | 23.6% |
| 30 | 10.3% | 54.0% | 58.3% | 35.8% | 4.5% | 0.7% | 5.4% | 490.1903 | 1.224 | ND | 20.1% | |||
| 14P | 1105 | 28 | 30 | 22.0% | 33.1% | 80.8% | 16.8% | 2.3% | 0.1% | 0.1% | 490.1902 | 1.020 | ND | 29.1% |
| 31 | 13.8% | 45.6% | 84.0% | 15.4% | 0.6% | 0.0% | 2.6% | 490.1883 | 2.856 | ND | 28.7% | |||
| 32 | 22.7% | 37.1% | 72.5% | 21.9% | 4.4% | 0.2% | 0.3% | 490.1908 | 2.244 | ND | 29.7% | |||
| 16P | 660 | 25 | 29 | 12.7% | 26.6% | 90.1% | 9.2% | 0.7% | 0.0% | 0.1% | 490.1908 | 2.244 | ND | 48.3% |
| 31 | 6.4% | 56.0% | 80.5% | 18.2% | 1.4% | 0.0% | 0.2% | 490.1892 | 1.020 | ND | 32.5% | |||
| 33 | 15.6% | 35.1% | 50.4% | 41.8% | 7.6% | 0.1% | 0.1% | 490.1900 | 0.612 | ND | 39.8% | |||
| 35 | 13.3% | 31.6% | 62.9% | 31.7% | 5.4% | 0.0% | 0.2% | 490.1902 | 1.020 | ND | 42.3% | |||
| 18P | 930 | 25 | 26 | 25.1% | 36.7% | 81.4% | 14.6% | 3.8% | 0.2% | 0.2% | 490.1904 | 1.428 | ND | 31.1% |
| 28 | 21.2% | 25.3% | 84.0% | 14.5% | 1.6% | 0.0% | 0.2% | 490.1899 | 0.408 | ND | 37.6% | |||
| 20P | 900 | 29 | 31 | 6.1% | 80.8% | 81.4% | 16.3% | 2.0% | 0.3% | 40.4% | 490.1894 | 0.612 | ND | 11.7% |
| 37 | 5.0% | 84.2% | 78.9% | 19.2% | 1.7% | 0.2% | 40.7% | 490.1898 | 0.204 | ND | 10.1% | |||
| 21P | 1060 | 29 | 31 | 10.5% | 67.9% | 86.0% | 11.8% | 2.0% | 0.1% | 29.8% | 490.1898 | 0.204 | 0.09% | 14.9% |
| 33 | 12.8% | 68.5% | 82.1% | 14.8% | 3.0% | 0.2% | 27.1% | 490.1882 | 3.060 | 0.04% | 14.7% | |||
| 22P | 490 | 23 | 27 | 11.7% | 70.0% | 71.4% | 26.1% | 2.2% | 0.3% | 34.3% | 490.1879 | 3.672 | ND | 13.3% |
| 26P | 1070 | 28 | 31 | 11.4% | 67.6% | 77.0% | 18.4% | 4.2% | 0.4% | 25.8% | 490.1899 | 0.408 | 0.05% | 16.7% |
| 33 | 9.8% | 70.8% | 76.1% | 18.3% | 5.0% | 0.5% | 23.5% | 490.1897 | 0.000 | 0.02% | 14.3% | |||
| 35 | 8.9% | 78.0% | 63.4% | 29.9% | 5.6% | 1.1% | 28.5% | 490.1908 | 2.244 | 0.11% | 12.8% | |||
| 36 | 9.1% | 62.4% | 69.8% | 27.0% | 3.2% | 0.0% | 0.2% | 490.1897 | 0.000 | 0.51% | 23.4% | |||
| 44 | 8.8% | 83.8% | 62.5% | 31.1% | 5.3% | 1.1% | 34.0% | 490.1907 | 2.040 | 0.36% | 7.7% | |||
| 27P | 940 | 28 | 31 | 11.8% | 58.9% | 73.3% | 25.3% | 1.4% | 0.0% | 0.1% | 490.1889 | 1.632 | 0.16% | 23.2% |
| 33 | 11.1% | 58.6% | 73.8% | 24.1% | 2.1% | 0.0% | 0.4% | 490.1891 | 1.326 | 0.37% | 20.8% | |||
| 35 | 8.5% | 61.3% | 69.9% | 23.9% | 2.1% | 0.3% | 10.4% | 490.1913 | 3.264 | 0.09% | 12.3% | |||
| 38 | 12.4% | 53.6% | 63.8% | 32.3% | 3.9% | 0.1% | 0.0% | 490.1898 | 0.102 | 0.44% | 30.7% | |||
| 41 | 7.7% | 56.3% | 59.9% | 34.7% | 5.2% | 0.2% | 0.1% | 490.1893 | 0.816 | 0.71% | 30.2% |