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Kinetics of protein accumulation after induction of tetO7-TATACYC1. Time-course experiments showing the accumulation, by western blotting, of (A) FLAG3-Top1 -and (B) FLAG3-Nse6 following induction of tetO7-TATACYC1 by addition of 2.5 μg mL 1ahTet to the growth medium. A yeast protein of unknown identity that cross-reacted with the anti-FLAG tag antibody (*) is used as a loading control.
