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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Yeast. 2012 Sep 12;29(10):425–434. doi: 10.1002/yea.2920

Figure 6.

Figure 6

tetO7-TATACYC1 can be induced during meiosis. Wild type and nse6Δ cells or a cross between the tetO7-nse6 and nse6Δ strains were stimulated to undergo meiosis in the presence or absence of 2.5 μg mL−1 ahTet. The resulting cell-ascus mixtures were: (A) imaged by microscopy to determine ascus morphology and the DNA content of spores by DAPI staining, and (B) processed to isolate individual spores whose viability was measured by plating them on rich medium. Relevant asci are highlighted by dashed white lines. Error bars indicate the standard error of the mean from three independent crosses. Crosses between the tetO7-nse6 and nse6Δ cells were carried out using both h+ (leftward set of bars in B) and h− (rightward set of bars in B) tetO7-nse6 strains and the matching nse6Δ mutants. BF = bright field, DNA = DAPI.