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. 1982 Dec;38(3):1301–1303. doi: 10.1128/iai.38.3.1301-1303.1982

Involvement of cytochrome b-245 in the respiratory burst of human neutrophils.

N Borregaard, E R Simons, R A Clark
PMCID: PMC347891  PMID: 7152673

Abstract

The cytochrome b 245 of human neutrophils was reduced when the neutrophils under anaerobic conditions were stimulated by serum-treated zymosan, immune complexes, or phorbol myristate acetate. Reintroduction of oxygen rapidly reoxidized the cytochrome; azide was without effect on any of these reactions. This indicates that cytochrome b 245 participates in the respiratory burst of neutrophils.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babior B. M., Kipnes R. S. Superoxide-forming enzyme from human neutrophils: evidence for a flavin requirement. Blood. 1977 Sep;50(3):517–524. [PubMed] [Google Scholar]
  2. Borregaard N., Herlin T. Energy metabolism of human neutrophils during phagocytosis. J Clin Invest. 1982 Sep;70(3):550–557. doi: 10.1172/JCI110647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borregaard N., Juhl H. Activation of the glycogenolytic cascade in human polymorphonuclear leucocytes by different phagocytic stimuli. Eur J Clin Invest. 1981 Aug;11(4):257–263. doi: 10.1111/j.1365-2362.1981.tb02114.x. [DOI] [PubMed] [Google Scholar]
  4. Borregaard N., Kragballe K. Role of oxygen in antibody-dependent cytotoxicity mediated by monocytes and neutrophils. J Clin Invest. 1980 Oct;66(4):676–683. doi: 10.1172/JCI109904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holmes B., Page A. R., Good R. A. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. doi: 10.1172/JCI105634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Root R. K., Metcalf J. A. H2O2 release from human granulocytes during phagocytosis. Relationship to superoxide anion formation and cellular catabolism of H2O2: studies with normal and cytochalasin B-treated cells. J Clin Invest. 1977 Dec;60(6):1266–1279. doi: 10.1172/JCI108886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
  10. Segal A. W., Garcia R., Goldstone H., Cross A. R., Jones O. T. Cytochrome b-245 of neutrophils is also present in human monocytes, macrophages and eosinophils. Biochem J. 1981 Apr 15;196(1):363–367. doi: 10.1042/bj1960363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Segal A. W., Jones O. T. Absence of cytochrome b reduction in stimulated neutrophils from both female and male patients with chronic granulomatous disease. FEBS Lett. 1980 Jan 28;110(1):111–114. doi: 10.1016/0014-5793(80)80035-4. [DOI] [PubMed] [Google Scholar]
  12. Segal A. W., Jones O. T. Novel cytochrome b system in phagocytic vacuoles of human granulocytes. Nature. 1978 Nov 30;276(5687):515–517. doi: 10.1038/276515a0. [DOI] [PubMed] [Google Scholar]
  13. Ward P. A., Zvaifler N. J. Quantitative phagocytosis by neutrophils. I. A new method with immune complexes. J Immunol. 1973 Dec;111(6):1771–1776. [PubMed] [Google Scholar]

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