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. 2012 Oct 22;209(11):2127–2135. doi: 10.1084/jem.20120408

Figure 1.

Figure 1.

MCs have specific expression of Tph-1. (A) Tph-1 expression (as determined by qRT-PCR) of the indicated cell types standardized to β-actin expression (mean ± SEM) is shown. For each cell type, two independent FACS-sorted samples were used. Each MC and macrophage sample was pooled from the peritoneal lavage of 20 mice. All other samples were pooled from the LN and spleen of two to four mice. (B) Peritoneal MC expression of the following genes was determined by qRT-PCR: arginase (Arg), dopa decarboxylase (Ddc), histidine decarboxylase (Hdc), IDO, inducible nitric oxide synthase (iNos), l-threonine dehydrogenase (Tdh), TDO, Tph-1, and Tph-2. Two independent samples were used to determine mean ± SEM shown. (A and B) ND indicates that a signal was not detected. (C) Intracellular staining for Tph-1 was performed for MCs, CD4+ T cells, CD8+ T cells, B cells, CD11chi DCs, and F4/80+ CD11b+ macrophages in WT (bold lines) and Tph-1−/− (shaded areas) mice (n = 3–4 mice/group, and each cell type was tested in at least two independent experiments). (D) Naive LN sections were sectioned and stained for Tph-1 expression on MCs. CD117 staining is blue, Tph-1 staining is green, and overlap is white. Results are representative of three different mice/group. Bar, 10 µm.