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. Author manuscript; available in PMC: 2012 Oct 23.
Published in final edited form as: J Cell Biochem. 2010 Dec 15;111(6):1531–1536. doi: 10.1002/jcb.22883

Figure 1.

Figure 1

The osteoclastogenic effects of PTH (30 nM) and calcitriol (100 nM) in UMR-106/RAW 264.7 cell co-cultures as assessed by TRAP activity are markedly attenuated when the UMR-106 cells stably express caGα12 or caRhoA constructs. Cells were seeded at a density of 104/ml for UMR-106 cells and 2,500/ml for RAW 264.7 cells in 96 well plates and maintained in α-MEM supplemented with 10% fetal bovine serum. Five-day treatments began on the day after seeding. * p<0.05 vs. co-cultures without PTH or calcitriol, + p<0.05 vs. co-cultures containing EV-transfected UMR-106 cells. N = 3.