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. Author manuscript; available in PMC: 2012 Oct 23.
Published in final edited form as: J Cell Biochem. 2010 Dec 15;111(6):1531–1536. doi: 10.1002/jcb.22883

Figure 3.

Figure 3

The Rho kinase inhibitor, Y27632, reverses the inhibitory effects of stable expression of caGα12 or caRhoA in UMR-106 cells on PTH (30 nM) + calcitriol (100 nM)-stimulated osteoclastogenesis in co-cultures with RAW 264.7 cells. Cells were seeded at a density of 104/ml for UMR-106 cells and 2,500/ml for RAW 264.7 cells in 96 well plates and maintained in α-MEM supplemented with 10% fetal bovine serum. Five-day treatments began on the day after seeding. * p<0.05 vs. no Y27632, + p<0.05 vs. response to PTH + calcitriol in co-cultures containing EV-transfected UMR-106 cells. N = 3.