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. Author manuscript; available in PMC: 2012 Oct 23.
Published in final edited form as: J Cell Biochem. 2010 Dec 15;111(6):1531–1536. doi: 10.1002/jcb.22883

Figure 4.

Figure 4

Stable expression of caGα12 or caRhoA in UMR-106 cells fails to differentially attenuate responses to RANKL (30 ng/ml) in UMR-106/RAW 264.7 cell co-cultures. In the same experiment, caGα12 and caRhoA attenuated the response to PTH (30 nM) + calcitriol (100 nM). Cells were seeded at a density of 104/ml for UMR-106 cells and 2,500/ml for RAW 264.7 cells in 96 well plates and maintained in α-MEM supplemented with 10% fetal bovine serum. Five-day treatments began on the day after seeding. * p<0.05 vs. co-cultures without PTH + calcitriol, + p<0.05 vs. co-cultures containing UMR-106 cells stably expressing dnRhoA or EV, $ p<0.05 vs. effect of RANKL in RAW 264.7 cells alone. N = 6.