Bioflavonoid antiviral activity. A. Effect of 25μM bioflavonoids on viral proliferation. Huh-7.5 cells were infected with the reporter virus and immediately treated with bioflavonoids. 48 hours post treatment, luciferase activity was assayed. B. Effect of bioflavonoids on Renilla activity. Luciferase assays were performed on cells transfected with pRL-TK plasmid. C. MTT assays performed in parallel with assays in parts A and B. D. Effect of 125μM silymarin on viral proliferation. Huh-7.5 cells were infected with the reporter virus and treated with bioflavonoids for 48 hours as in part A, followed by luciferase assay. (*, **, and *** indicate P<0.05, P<0.005, and P<0.0005, respectively. Error bars reflect standard deviation.)