Figure 2.

Conserved sequence blocks from multiple sequence alignment of KivRFp from Fusarium proliferatum LF061 and other related proteins. Sequences alignment was carried out with CLUSTALW [25] and BoxShade Server ( http://www.ch.embnet.org/software/BOX_form.html). XP_382715, hypothetical protein from Gibberella zeae PH-1; ACI30654, ketoisovalerate reductase from Beauveria bassiana ATCC 7159; P0A9J4, PanE ketopantoate reductase from E. coli. The alignment was optimized and annotated with secondary structure predictions in SWISS-MODEL [32] by use of the determined crystal structure of PanE (2OFP, chain A). Identical amino acids are shown on black ground. Key amino acid residues mentioned in the text are labeled with arrows and numbered with reference to the PanE sequence. The nucleotide binding signatures and the hinge region separating the N-terminal Rossmann fold and the C-terminal catalytic domain in PanE are boxed [30,33].