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SDS-polyacrylamide gel of overexpressed ketoisovalerate reductase KivRFp in E. coli . Lane M: molecular weight protein marker (TransGen, Cat. No: DR301); lane 1, induced culture of E. coli/pET28a: total protein extract, as negative control; lane 2, total protein extract, induced culture of E. coli/ pKivRFp-His; lane 3: purified KivRFp (52 kDa).
