Table 1.
Comparison of primers and genomic coordinates used in this study for methylation-specific PCR in comparison to Herman et al.[7]
| Position/AB060808.1 | Position / Start codon | Sequence | ||
|---|---|---|---|---|
|
CDS p16/CDKN2A exon 1 |
192221- > 192072 |
0 - > 149 |
ATGGAGCCGG…..GCCGATCCAG |
|
|
This study |
F1 |
192197- > 192176 |
24 - > 45 |
ATGGAGCCTTCGGCTGACTGGC |
| |
R1_primer |
192050- > 192033 |
171 - > 188 |
CCCGCCATCCCCTGCTCC |
| |
R2_primer |
192077- > 192060 |
144 - > 161 |
CCCTCTACCCACCTGGAT |
|
Herman |
F1 |
192301- > 192278 |
−80 - > −57 |
TCACCAGAGGGTGGGGCGGACCGC |
| R1 | 192172- > 192151 | 49 - > 70 | TTAACAAAAAAAAAAAACTAAACTCCTC | |