Table 1. Tsal-binding kinetic parameters.
| Tsal1 | Tsal2A | |||||
| ka (M−1s−1) | kd (s−1) | KD (nM) | ka (M−1s−1) | kd (s−1) | KD (nM) | |
| dsDNA | 1.5E+06 | 6.7E−04 | 0.45 | 1.5E+06 | 6.9E−04 | 0.46 |
| dsDNA 25bp. | 4.6E+05 | 9.1E−04 | 2.0 | 6.5E+05 | 1.2E−03 | 1.9 |
| dsDNA 25bp. polyA/T | 1.0E+06 | 1.0E−03 | 1.0 | 1.1E+06 | 1.4E−03 | 1.3 |
| dsDNA 25bp. polyG/C | 2.8E+04 | 1.4E−03 | 49 | 6.2E+04 | 1.2E−03 | 19 |
| dsDNA 25bp. poly(AT) | 1.6E+04 | 1.4E−03 | 85 | 4.1E+04 | 1.3E−03 | 31 |
| dsDNA 25bp. poly(GC) | 4.0E+04 | 1.6E−03 | 39 | 4.7E+04 | 2.0E−03 | 42 |
| dsRNA | 5.8E+05 | 1.6E−03 | 2.8 | 1.4E+06 | 2.4E−03 | 1.7 |
| ssDNA 25nt. | 3.2E+04 | 2.0E−03 | 64 | 2.2E+04 | 1.8E−03 | 85 |
| ssDNA 25nt. polyA | 4.9E+04 | 7.7E−04 | 16 | 5.6E+04 | 9.1E−04 | 16 |
| ssDNA 25nt. polyT | 7.8E+03 | 2.2E−03 | 281 | 1.4E+04 | 1.2E−03 | 87 |
| ssDNA 25nt. polyG | 5.0E+04 | 1.4E−03 | 27 | 6.0E+04 | 1.3E−03 | 21 |
| ssDNA 25nt. polyC | 1.7E+04 | 7.9E−04 | 46 | 1.8E+04 | 1.4E−03 | 79 |
| ssDNA 25nt. poly(AT) | 1.1E+04 | 1.8E−03 | 162 | 1.9E+04 | 1.7E−03 | 91 |
| ssDNA 25nt. poly(GC) | 1.3E+04 | 1.9E−03 | 151 | 2.3E+04 | 1.8E−03 | 77 |
Tsal-binding kinetic parameters (ka, kd and KD) for the different tested nucleic acid analytes determined using surface plasmon resonance with 1000 RU recombinant Tsal1 and Tsal2 immobilized onto a CM5 sensor chip. Sensograms were fitted for a Langmuir 1∶1 binding model with local Rmax using the BIA-evaluation software version 4.1.