Table I.
Potassium Salt | Net
Ca2+ Uptake
|
||
---|---|---|---|
Total | Pump | Antiport | |
nmol/mg (-fold) | |||
None | 1.92 (1.00) | 0.98 (1.00) | 0.94 (1.00) |
+Potassium oxalate | 5.00 (2.63) | 1.04 (1.06) | 3.96 (4.21) |
+Potassium phosphate | 3.95 (2.06) | 0.90 (0.92) | 3.05 (3.25) |
Net ATP-dependent 45Ca2+ uptake at 6 min was determined with 0.1 μm Ca in the reaction mixture as described in Figure 5. Uptake was measured in the absence (Total) or presence (Pump) of 5 μm gramicidin and 0.5 μm bafilomycin. Antiport activity was determined as the difference between “total” and “pump” activity. Potassium oxalate or potassium phosphate was added to a final concentration of 10 mM. Data represent the averages of two experiments.