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. 2012 Oct 23;7(10):e47070. doi: 10.1371/journal.pone.0047070

Figure 1. Apoptotic bodies significantly reduce inflammatory respiratory burst responses of goldfish primary macrophages.

Figure 1

(A) Goldfish PKM were separately incubated with apoptotic bodies or zymosan (5∶1, particle: cell ratio) for 2 h. Cells were then harvested and respiratory burst was assayed using DHR. n = 3. (B) Goldfish PKM were cultured in the presence of both apoptotic bodies and zymosan for 2 h (5∶1 ratio for both). Respiratory burst was then analyzed in cells based on phagocytic capacity as follows: non-phagocytic cells, cells containing only apoptotic bodies, cells containing only zymosan or cells containing both apoptotic bodies and zymosan (white bars). Representative images are shown for cells in each of these groups. To investigate the effects of pre-incubation with apoptotic bodies, apoptotic bodies were added to PKM simultaneously to zymosan (‘−0 h’), 2 h before zymosan (‘−2 h’) or 4 h before zymosan (‘−4 h’). n = 4. For all, * p<0.05; ** p<0.01; For (c), + p<0.05 and ++ p<0.01 compared to (‘−0 h’). No- no internalized particle; AB- apoptotic body; Zy- zymosan.