Figure 2. In vivo administration of zymosan induces a marked infiltration of leukocytes that is linked to high levels of respiratory burst.

Goldfish (left) and C57BL/6 mice (right) were injected intraperitoneally with 2.5 mg of zymosan. Cells were harvested by peritoneal lavage at 0 h (saline alone), 8, 24 and 48 h and counted (top row). Injection of zymosan resulted in a marked increase in cell numbers isolated from the peritoneum that peaked at 48 h for mice and 24 h for goldfish. Respiratory burst in isolated cells at these time points was determined with DHR (bottom row). n = 4; * p<0.05; ** p<0.01.