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. 2012 Oct 23;7(10):e47070. doi: 10.1371/journal.pone.0047070

Figure 3. Pro-inflammatory (zymosan) and homeostatic (apoptotic bodies) stimuli differentially impact leukocyte infiltration profiles in goldfish and mice.

Figure 3

Goldfish and C57BL/6 mice were injected intraperitoneally with saline, apoptotic bodies (5×106) or zymosan (2.5 mg). Apoptotic bodies were also pre-injected 4 h before zymosan injections. Goldfish leukocyte populations were defined by imaging flow cytometry (area, internal complexity, and morphology) and staining patterns with Sudan Black and an anti-CSF-1R antibody (Figure S3). Murine cells were defined based on surface markers for neutrophils (F4/80−/Gr1+/CD11b+), monocytes (F4/80lo/Gr1+/−/CD11b+), macrophages (F4/80hi/Gr1+/−/CD11b+) and lymphocytes (F4/80−/Gr1−; CD3, B220, NK1.1; Figure S4A). n = 4; * p<0.05 and ** p<0.01 compared to control; + p<0.05 and ++ p<0.01 compared to zymosan. No- no internalized particle; AB- apoptotic body; Zy- zymosan.