Figure 7. Apoptotic bodies downregulate murine neutrophil ROS production in a contact dependent manner.

Goldfish (left) and C57BL/6 mice (right) were injected intraperitoneally with zymosan (2.5 mg). Activated peritoneal cells from were harvested by peritoneal lavage and subpopulations were isolated by density centrifugation. (A) Separated neutrophil or mononuclear populations were incubated with labeled apoptotic bodies for 2 h and internalization was analyzed. n = 4; * p<0.05 and ** p<0.01; Mφ/M  =  macrophage/monocyte. (B) Isolated populations were cultured for 2 h in the presence of the indicated stimuli. Conditions denoted within brackets were contained within a 4 μm transwell. After 2 h, responder cells outside the transwells were harvested and respiratory burst was assayed using DHR.