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. 2012 Oct 23;7(10):e47888. doi: 10.1371/journal.pone.0047888

Figure 3. Northern blot analysis.

Figure 3

The effects of insertion of the silencing vectors pSilent1-endochi and pSilent1-ToxA on the expression of three chitinase genes (Tvech1, Tvech2 and Tvech3) in T. virens transformants are shown in (a) detection of Tvech1 transcripts, (b) detection of Tvech2 transcripts and, (c) detection of Tvech3 transcripts. Transformants containing pSilent1-endochi vector have the prefix S.pNO and the ones containing pSilent1-ToxA have the prefix S.pOf. Twenty micrograms of total RNA were separated on formaldehyde-agarose gels, transferred to nylon membranes and probed with a 1 kb gene-specific fragment. Equal loading of total RNA was estimated by ethidium bromide staining of rRNA.