Table 1. Details of PCR primers used in this study.
| Primer name | Sequence 5′-3′ | Target |
| Tvech1-Fw | Cagcacagaagtggcaagcttgaa | Tvech1 gene |
| Tvech1-Rv | acttggtacacacacgaattcacc | Tvech1 gene |
| Tvech1-Rv2 | tccgttgtaagtctggccaatacc | Tvech1 gene |
| Tvech2-Fw | ccatctcaagtaaacatgtcggga | Tvech2 gene |
| Tvech2-Rv | gggtgcaaacaacaagtaagcctc | Tvech2 gene |
| Tvech2-Rv2 | ttgtgggttatagcagtagctgga | Tvech2 gene |
| Tvech3-Fw | gaccagaacctactttgaaggctt | Tvech3 gene |
| Tvech3-Rv | gcctcgcagaaccatacaagacaa | Tvech3 gene |
| Tvech3-Rv2 | ttgcccagatacagtatcccagct | Tvech3 gene |
| Tvnag1-Fw | cgtagtagtgctattgccatcgct | Tvnag1 gene |
| Tvnag1-Fw2 | gatactacccagacgttcaagccg | Tvnag1 gene |
| Tvnag1-Rv | tcccggcttctcttaatccatacc | Tvnag1 gene |
| Tvnag1-Rv2 | acggccgcagtccaagtactataa | Tvnag1 gene |
| Tvnag2-Fw | Tcgtgtggcctgaccttgtcaact | Tvnag2 gene |
| Tvnag2-Fw2 | tcttcttcaagcatagctcaggca | Tvnag2 gene |
| Tvnag2-Rv | taccacactgcatactttgttccg | Tvnag2 gene |
| Tvnag2-Rv2 | ataaccttgaatccagcctccgcg | Tvnag2 gene |
| HEX1_Fw | ccggttgctgccgaaaccttcaat | Tvnag2 LF |
| HEX1.M13F_Rev | gtcgtgactgggaaaaccctggcggttgccggttgaggagtgtttcgg | Tvnag2 LF |
| HEX2.M13R_Fw | tcctgtgtgaaattgttatccgctccgattacgcagctgtggtgtagt | Tvnag2 RF |
| HEX2_Rev | ggccgaaatgatgaccctttgcgg | Tvnag2 RF |
| Chito2_50.1 | gcttcgactgctactgtactccgt | Tvnag1 LF |
| Chito2_m13F | gtcgtgactgggaaaaccctggcggctgaaagccaatgcggcaatcgc | Tvnag1 LF |
| Chito2_m13R | tcctgtgtgaaattgttatccgctcgctgttcacctaaggggagagtc | Tvnag1 RF |
| Chito2_30.1 | aacagcatacactgggaagactcg | Tvnag1 RF |
| HY split | ggatgcctccgctcgaagta | hph gene |
| YG split | cgttgcaagacctgcctgaa | hph gene |
| IL split | tctggttgtattctcaggac | ilv gene |
| LV split | cataccaagcatgtgcagtg | ilv gene |
| M13 F | cgccagggttttcccagtcacgac | binding site of pUC/M13 forward sequencing primer |
| M13 R | agcggataacaatttcacacagga | binding site of pUC/M13 reverse sequencing primer |
| 1F.endochi.XhoI | cgccaaatctagtctcgagct | Tvech2 gene |
| 2R.endochi.HindIII | aagcttacccctccccgatgccattat | Tvech2 gene |
| 3F.endochi.KpnI | ggtacccgccaaatctagtctcgagct | Tvech2 gene |
| 4R.endochi.SphI | gcatgcacccctccccgatgccattat | Tvech2 gene |
| 1F.SpeI.ToxA | actagtcatggaggagttctgtacgcgc | ToxA promoter |
| 2R.fusion.ToxA | agcagctcgagactagatttggcggacctatattcattcaatgtcagc | ToxA promoter |
| 3F.fusion.ToxA | gctgacattgaatgaatataggtccgccaaatctagtctcgagctgct | Tvech2 gene |
| Hex probe F | accagacggtccaagttacctaca | Tvnag2 gene |
| Hex probe R | tgtttgccttatactcgtcgcctc | Tvnag2 gene |
| Chito2 probe F | cgtgctcttcattgaccaggctgt | Tvnag1 gene |
| Chito2 probe R | gctcggagtcgttgacgttgagct | Tvnag1 gene |
| Hyg probe 2F | aagcctgaactcaccgcgacgtct | hph gene |
| Hyg probe 3R | tgctggggcgtcggtttccactat | hph gene |
| Endochi probe1F | tgagcttcctcggcaaatccgtgg | Tvech1 gene |
| Endochi probe1R | agcagaatccgtgttgaagggagt | Tvech1 gene |
| Endochi probe2F | gggagacggctatcgttcagttgc | Tvech2 gene |
| Endochi probe2R | cgaagatgccattctcccacgacc | Tvech2 gene |
| Endochi probe3F | gctcctttgcgtcgttttggccgt | Tvech3 gene |
| Endochi probe3R | gtgtcgccacggtcagaaggtagt | Tvech3 gene |
LF – left flank.
RF – right flank.