Figure 5. Activation of mouse NKT cells by CD1d orthologs.

Activation of mouse iNKT hybridomas 1.2 by recombinant human, mouse and bovine CD1d in a CD1d-coated plate assay, using either αGalCer (top) or C₁₆-αGalCer (C16, bottom). Individual amino acid mutations in CD1d are indicated. Concentrations of glycolipids used to load plate bound CD1d ranges from 0–5 µg/ml as indicated. iNKT cell activation is measured by the release of IL-2 in an ELISA assay. Experiment was performed twice, while measuring each condition in triplicate.