Figure 3.

Editing at several sites in the putative intronic stem loop. (A) Chromatogram showing A-to-I editing in the intronic stem of the Gabra-3 transcripts derived from mouse brain. US indicates the upper strand, while LS equals the lower strand according to the cartoon in B. Arrows indicate the edited adenosines. (B) Cartoon showing the sequence of the edited part in the intronic hairpin. Arrows indicate the edited adenosines. (C) Sanger sequencing chromatograms showing I/M editing of the wild-type Gabra-3 editing reporter in HeLa cells compared with a reporter where 9 edited adenosines were mutated to guanosines (9A->G) and a reporter with compensatory mutations, restoring the base pairs in the intronic stem (9A->G+9U->C).