Figure 4.

The intronic hairpin can induce editing at the I/M site prior to splicing. (A) Co-expression of the Gabra-3 exon–intron–exon constructs with the ADAR2 expression vector in HEK 293 cells. The chromatograms after sequencing show that the editing efficiency at the I/M site decrease from high to medium in the absence of the intronic editing inducer when spliced. (B) Detection of spliced and non-spliced Gabra-3 exon–intron–exon products after RT-PCR from the same samples as in (A). Deletion of the editing inducer (Δ149 exon–intron–exon) reduces the splicing efficiency compared to the WT exon–intron–exon reporter.