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. 2012 Jul 30;40(19):9876–9886. doi: 10.1093/nar/gks691

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Deletion analyses to determine the shortest sequence for efficient I/M editing of Gabra-3. Editing reporter constructs (A–E) expressing exon 9 and part of intron 9 were co-transfected into HEK 293 cells together with an ADAR1 or ADAR2 expression vector. The chromatograms after sequencing of the RT-PCR products at the I/M site are shown on top. Below are the putative secondary structures that can be formed at the I/M site. The I/M site is indicated in grey. (C′) indicates the same construct as in (C) but where 149 nt of the intronic hairpin is deleted. The number of deleted nucleotides within each reporter construct is shown below the putative structures.