Figure 2.
(A) Binding of the HhH motif mutants to a gapped DNA. The experiment was carried out as described in ‘Materials and Methods’ section, incubating 5 nM of 2-nt gapped DNA with 1 µM of either the wild-type or the indicated mutant PolXBs for 10 minutes at 4°C. The bars chart is the result of three independent experiments. (B) DNA polymerization activity on gapped DNA of HhH motif mutants. The assay was carried out as described in ‘Materials and Methods’ section, incubating 1.5 nM of a 2-nt gapped DNA with 125 nM of either the wild-type or the indicated mutant PolXBs in the presence of 8 mM of MgCl2 and 50 µM of dNTPs at 30°C. The positions corresponding to the non-extended primer and to the filling-in products are indicated.