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AP-endonuclease activity of the HhH-motif mutants. The assay was carried out as described in ‘Materials and Methods’ section, incubating 1.5 nM of a 34mer double stranded oligonucleotide containing in the 5′-labelled strand an internal THF (H) group, with 64 nM of either the wild-type or the specified mutant PolXBs in the presence of 1 mM of MnCl2 for the indicated period at 30°C.
