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. 2012 Jul 25;40(19):9850–9862. doi: 10.1093/nar/gks705

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Deep-sequencing analysis of small RNAs associated with endogenous Ago proteins. (A) Composition of the small RNA libraries from the input sample and immunoprecipitated Ago1, Ago2, Ago3, Ago4 and control (an unrelated antibody against the complement system protein RmC from rat). (B) Table of the 10 most abundant miRNAs associated with Ago1, Ago2 or Ago3. The colors indicate the same miRNA. (C) Comparison of the sorting profile from the deep-sequencing data (right side) for miR-182 (top panel), miR-92a (middle panel, right) and miR-30a (lower panel) with profiles resulting from northern blotting (left side).