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. 2012 Aug 2;40(19):9441–9454. doi: 10.1093/nar/gks720

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Nucleolin is associated with active rDNA repeats. (A) ChIP input DNA and DNA precipitated by nucleolin or UBF antibody were digested with HpaII or MspI, or they were mock-digested. The relative levels of HpaII-resistant, methylated rDNA copies (grey bars) and unmethylated copies (white bars) were determined by QPCR with the pair of primers that flanks the HpaII/MspI site on the rDNA promoter as indicated in Figure 2A. (B) and (C) co-immunoprecipitation of endogenous nucleolin with active and repressive histone modification marks on coding (H8 primers) and 5′-end of the coding region (H42.9 primers) regions of rDNA, respectively. Re-ChIP experiments with anti-H3K4me3, H3K9me2 and H4K12Ac antibodies were performed after a first immuno-precipitation with anti-nucleolin antibody. Values are means ± SD (standard deviation) derived from three independent experiments, each tested by at least three independent QPCR reactions.