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. 2012 Aug 9;40(19):9802–9814. doi: 10.1093/nar/gks728

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Single-turnover unwinding kinetics at different ATP concentrations. A total of 2 nM DNA substrate (16-bp duplex with a 15-nt 3′ tail) with 30 nM BLM, ATP of varying concentrations with 2.5 µM dA₈ and 2 µM DNA trap, and 5 µM dT₅₆ were first preincubated, respectively, and the reaction was initiated by rapid mixing of the three solutions. (A) Typical kinetic time-courses. The solid lines are best fits of the data to Equation (1) with n = 6. (B) Observed unwinding rate constant. The line corresponds to a best fit of the data to the Hill equation, , where h is the Hill coefficient. The fitted values are h = 0.92 ± 0.06 and K_M = 20.4 ± 1.7 µM.

Inline graphic — Single-turnover unwinding kinetics at different ATP concentrations. A total of 2 nM DNA substrate (16-bp duplex with a 15-nt 3′ tail) with 30 nM BLM, ATP of varying concentrations with 2.5 µM dA₈ and 2 µM DNA trap, and 5 µM dT₅₆ were first preincubated, respectively, and the reaction was initiated by rapid mixing of the three solutions. (A) Typical kinetic time-courses. The solid lines are best fits of the data to Equation (1) with n = 6. (B) Observed unwinding rate constant. The line corresponds to a best fit of the data to the Hill equation, , where h is the Hill coefficient. The fitted values are h = 0.92 ± 0.06 and K_M = 20.4 ± 1.7 µM.