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. 2012 Aug 9;40(19):9802–9814. doi: 10.1093/nar/gks728

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Unwinding of a 15-nt 3′-tailed DNA with varying duplex lengths. The measurements were performed under the same conditions as in Figure 4 except for that the ATP concentration was fixed at 1 mM. (A) Kinetic time courses. The solid lines are best fits of the data to Equation (1) with n = 4, 5, 6, 7 and 8 for the five data curves, respectively, with the corresponding values of k_obs as 11.7 ± 0.1, 12.5 ± 0.1, 15.5 ± 0.1, 12.0 ± 0.2 and 13.6 ± 0.2 s⁻¹. (B) Number of steps for unwinding the different duplexes. The straight line was a straight line with a slope of 0.5 bp⁻¹, corresponding to a step size of 2 bp.