Table 1.
Parameters for the full-length BLM and the BLM helicase core (amino acids 642–1290) in the absence or presence of ATP and DNA obtained from dynamic light scattering measurements
| Cofactor | Full-length BLM |
BLM helicase core |
||||
|---|---|---|---|---|---|---|
| % Mass | Radius (nm) | Calculated mass (kDa)a | % Mass | Radius (nm) | Calculated mass (kDa)b | |
| apo | 97.2 | 10.1 | 754 | 99.6 | 3.3 | 59.8 |
| DNA | 96.1 | 9.9 | 720 | 99.4 | 3.5 | 60 |
| ATP | 57.8 | 12.7 | 1127 | 99.3 | 2.8 | 34 |
| 42.2 | 4.5 | 112 | ||||
| DNA+ATP | 94.4 | 6.5 | 268 | 99.1 | 3.2 | 48 |
| AMPPNP | 90.5 | 20.3 | 2620 | 98.5 | 3.5 | 60 |
| 9.5 | 4.7 | 108 | ||||
| DNA+AMPPNP | 62.2 | 12.6 | 962 | 98.1 | 3.5 | 60 |
| 37.8 | 8.2 | 380 | ||||
aThe molecular weight of BLM is 159 kDa.
bThe molecular weight of the BLM helicase core is 74.1 kDa.