Figure 3.

The relative amount of LHCII in the stroma-exposed regions of thylakoid membranes isolated from spinach leaves during their acclimation from low- to high-intensity light. Stromal lamellae vesicles were isolated by digitonin incubation of thylakoid membranes at different stages in the acclimation process, followed by differential centrifugation. The relative amounts of LHCII were determined by mild SDS-PAGE. The light/dark cycle was 10 h of light followed by 14 h of darkness. The 100% value represents the initial amount of LHCII in the stromal thylakoids before exposure of plants to high light. Results are the means of three separate experiments.