Figure 4.
Correlation between LHCII phosphorylation and degradation. Thylakoid membranes were labeled using [γ-32P]ATP. The maximal amount of phospho-LHCII obtained at 160 μE m−2 s−1 for 15 min was taken as 100% phosphorylation. The extent of degradation was determined as a decrease in the LHCII-25/LHC-27 ratio during a 6-h period. The analysis was with two-dimensional SDS-PAGE combined with autoradiography and Coomassie blue staining. Results are from two independent experiments (expt1 and expt2).