Figure 5.

Analysis of the chloroplast proteases Clp and FtsH in connection with LHCII degradation. A, The presence of ClpA and ClpP in chloroplasts (lanes 1 and 2) and thylakoids (lanes 3 and 4) isolated from high-light (lanes 2 and 4) and low-light (lanes 1 and 3) spinach leaves was analyzed by SDS-PAGE and western blotting. B, Association of FtsH with thylakoid membranes during acclimation was investigated by washings with either NaBr or NaOH. Analysis was with SDS-PAGE and western blotting. Lane 1, Unwashed thylakoids; lane 2, NaBr pellet; lane 3, NaBr-wash supernatant; lane 4, NaOH pellet; lane 5, NaOH-wash supernatant.