Figure 9.

Top1 contributes to footprinting profiles around ROREs. NIH3T3 cells were incubated with 5 µM camptothecin or siRNAs (Cont, negative control; Top1, topoisomerase I) and analyzed by LM–PCR 12 h later. Arrow and arrowhead in the map of the Baml1 promoter, labeled probe and Top1-binding sites, respectively. Circles and triangles, bands that disappeared in presence of camptothecin plus Top1 siRNA, and in that of camptothecin alone, respectively. Arrow, Top1-binding site at position −58. Numbers beside marker indicate nucleotide position of Bmal1 gene.