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SDS-PAGE and Western blot analysis of house dust mite extracts. Allergen extracts were separated on 12% SDS-PAGE gels under reducing conditions (A) and probed with a monoclonal antibody raised against recombinant Der f 2 (B). M, molecular mass standard; KF, D. farinae extract standardized in Korea; UF, D. farinae extract standardized in USA; KP, D. pteronyssinus extract standardized in Korea; UP, D. pteronyssinus extract standardized in the US.
