Table II.
Day | Diurnal Period | Length | Total (15NH4+ + 14NH4+) Uptake | True 14NH4+ Release | True Root 14N Releasea |
---|---|---|---|---|---|
h | μmol plant−1 | period−1 | % | ||
7–8 | Dark | 10 | 49.8 | 6.9 | 4.0 |
8 | Light | 14 | 103.1 | 11.6 | 6.7 |
8–9 | Dark | 10 | 63.9 | 0.2 | 0.1 |
9 | Light | 14 | 177.3 | 11.1 | 6.4 |
9–10 | Dark | 10 | 90.6 | 5.2 | 3.0 |
10 | Light | 14 | 193.3 | 8.3 | 4.8 |
Total | 678.0 | 43.3 | 25.0 |
Roots of maize seedlings were exposed continuously to highly labeled 15NH4+ for 3 d. NH4+ flux rates were calculated from periodic analysis of the uptake solution, as illustrated in Table I.
14NH4+ release of the total 14N present in roots harvested on the 7th d (174 μmol 14N root−1).