Figure 1.
Expression of β1 and β2Adrenergic Receptors in Pancreatic Cancer Cell Lines
Human pancreatic cells AsPC-1, BxPC-3, Capan-2, HPAF-II, MIA PaCa-2 and Panc-1 were propagated to confluence and extracted for RNA and protein. (A) Total RNA extracts were used to determine mRNA expression levels of β1AR and β2AR. (B) Total protein were resolved by PAGE, transferred on PVDF membrane and probed for β1AR or β2AR specific antibody and visualized with HRP-conjugated secondary antibody and chemiluminescence. Antibody specific for β-actin was used as visual loading control. Data are presented as relative expression ± SEM based on band densitometry normalized β-actin. Statistical significance of p<0.05 are indicated by the asterisk (*) for comparison of β1AR data sets and the double asterisks (**) for comparison of β2AR data sets among the cell lines only.