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. Author manuscript; available in PMC: 2013 Dec 10.
Published in final edited form as: Life Sci. 2012 Jun 19;91(23-24):1153–1158. doi: 10.1016/j.lfs.2012.06.004

Fig. 1. Greater relative increase of Aβ in cell lysate compared to conditioned media of Swedish mutant APP compared to wild-type human APP expressing N2a cells.

Fig. 1

A. Representative blot of Aβ (lowest molecular weight bands) showing a greater relative increase in cell lysates (right blot) compared to conditioned media (left blot) in Swedish mutant human APP (“SWE”) compared to wild-type human APP (“WT”) expressing N2a cells using IP and Western blot for Aβ detection. Note the equivalent amounts of full-length APP in cell lysates, which also provides evidence that cell confluence was similar for the conditioned media. 4G8 antibody was used for immunoprecipitation; 6E10 antibody was used for immunoblotting. B. Lack of decrease in Aβ levels using direct load Western blot of lysates from Swedish mutant APP N2a cells after trypsin treatment, which is similar to untreated cells (CTRL), supports the intracellular localization of this Aβ. n=3.