Skip to main content
. Author manuscript; available in PMC: 2013 Dec 10.
Published in final edited form as: Life Sci. 2012 Jun 19;91(23-24):1153–1158. doi: 10.1016/j.lfs.2012.06.004

Fig. 3. Addition of Aβ in the cell medium induces generation of Aβ inside cells.

Fig. 3

A. Addition of conditioned medium from Swedish mutant APP N2a cells (SWE, right lane) induces an increase of intracellular Aβ in wild-type (WT) cells compared to medium from wild-type cells (center lane). The detected Aβ is not likely to primarily come from the medium via internalization, since N2a cells treated with the SWE medium do not show a band (left lane). 4G8 antibody was used for immunoprecipitation; 6E10 antibody was used for immunoblotting. n=3, **p<0.01. B. Metabolic labeling demonstrates an increase in newly generated Aβ in Swedish mutant APP N2a cells in proportion to the concentration of synthetic human Aβ1–42 (10 or 25μM) added to the medium.