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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Int J Cancer. 2012 May 14;131(12):2820–2831. doi: 10.1002/ijc.27598

Figure 2.

Figure 2

Expression profiles of BMAL1 and CLOCK genes over 24 hr in ACC-MESO-1 and MeT-5A cells. qRT-PCR analysis of BMAL1 and CLOCK mRNA in ACC-MESO-1, MeT-5A at time indicated above. NIH3T3 cells were used as a positive control. Serum shock was performed as mentioned in the “Materials and Methods” Section. We found that serum shock induces rhythmical expression changes of BMAL1 and CLOCK mRNA in NIH3T3 as well as MeT-5A cells. Serum shocked ACC-MESO-1 cells showed rhythmical changes of CLOCK mRNA, but not BMAL1 (GAPDH was used as an internal control). The results are averages of two independent experiments done in duplicate.