Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Invest Dermatol. 2012 Jul 19;133(1):31–41. doi: 10.1038/jid.2012.230

Figure 1.

Figure 1

Wntless expression correlates with Wnt activation during anagen. (a) Wildtype (P60) club hairs were left either unplucked (telogen) or plucked to induce anagen and harvested at either 2 days (Ana I), 3 days (Ana II), 4 days (Ana III), or 9 days (Ana VI) post-depilation. Sections were analyzed for Wls expression by immunofluorescence. (b) Brightfield immunohistochemical detection of β-catenin during telogen and anagen phases as described above. Cells positive for nuclear β-catenin (arrowheads) in epithelium and DP. (c) In situ hybridization detection of Axin2 mRNA transcripts was used to detect Wnt activation on cryosections of telogen and depilation-induced early anagen phase hair follicles. (d) Illustrations of the temporospatial distribution of Wls expression and Wnt activation during telogen and anagen phases. Bar=50Om.