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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Invest Dermatol. 2012 Jul 19;133(1):31–41. doi: 10.1038/jid.2012.230

Figure 4.

Figure 4

HFSCs require Wls to promote Wnt/β catenin signaling and anagen phase. (a) Mutant and control mice were shaved at P20 and RU486 was applied topically for 6 days. Skin was analyzed during anagen (P33-P40). (b) Control mice grew hair over shaved area while mutant skin remained bare by P40. (c) H&E histology of control and mutant skin at P40. (d) Corresponding morphological hair cycle stage analysis of control and mutant hair follicles. (e) Ki67 immunohistochemistry of Wls K15cKO hair follicles compared to control depilation-induced early anagen and telogen follicles (P33). (f) HFSC markers K15 and S100A4 are expressed normally within the bulge/sHG of Wls K15cKO follicles (P40). (g) β-catenin immunohistochemistry shows a lack of nuclear β-catenin in the sHG and DP of arrested mutant hair follicles, similar to control telogen follicles (white bars=50μm, black bars=100μm).