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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Invest Dermatol. 2012 Jul 19;133(1):31–41. doi: 10.1038/jid.2012.230

Figure 5. Epidermal Wnt ligand secretion is required for hair follicle neogenesis following wound healing.

Figure 5

Epidermal Wnt ligand secretion is required for WIHN. (a) LEF-1, a marker of Wnt/β-catenin signaling, is expressed in the epithelium and mesenchyme of germ and peg stage neogenic hair follicles from wildtype mice. (b) β-catenin immunohistochemistry of WIHN in wildtype mice taken just after reepithelialization, prior to hair neogenesis. Nuclear β-catenin was not detected in either the epidermis or dermis. (c) Schematic of tamoxifen induction and wounding. (d) whole-mount AP staining of dermal wound preps from control and Wls K14cKO mice (top), whole-mount K17 staining of underside of epidermal sheets (middle), and H&E histology of wound sections (bottom; bar=100μm). Control and mutant whole-mounts were taken at same magnification. (e) Graphical distribution of number of neogenic hair follicles per wound in control and mutant mice determined by counting AP+ spots from dermis AP whole mounts (bar represents mean).