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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Dev Biol. 2012 Aug 28;372(1):103–110. doi: 10.1016/j.ydbio.2012.08.013

Fig. 2.

Fig. 2

Eif4a3 morphant muscle cells are insensitive to caffeine, and contract in response to the calcium ionophore Ionomycin. (A) Eif4a3 knockdown inhibits caffeine-mediated contraction in muscle cell cultures. Muscle cells were prepared from Eif4a3MM (MM)- or Eif4a3MO- injected stage 23 embryos and treated with 25mM caffeine. (B) Graph summarizing caffeine experiment results. (C) Ionomycin induces contractility in both wild-type and Eif4A3 morphant cell cultures. Muscle cells were prepared from Eif4a3MM (MM)- or Eif4a3MO- injected stage 23 embryos and treated with 2uM Ionomycin. (D) Graph summarizing Ionomycin experiment results.