Fig. 3.

Eif4a3 knockdown leads to a reduction in ryanodine receptor (ryr) RNA and protein levels. (A) Semi-quantitative RT-PCR analysis of ryr (25 cycles); odc was used as a loading control. RNA was purified from stage 27 embryos injected with mismatch control morpholinos (MM), Eif4a3 morpholinos (Eif4a3MO), or co-injected with Eif4a3MO and eif4a3 RNA (Rescue). (B) Western blot analysis with anti-Ryr antibody (34C, Developmental Studies Hybridoma Bank). β-Tubulin, visualized with an anti-β-Tubulin antibody, was used as an internal control.